Light Scattering Studies on Supersaturated Protein Solutions

Light Scattering Studies on Lecithin Micellar Solutions

Protein interactions in undersaturated and supersaturated solutions: a study using light and x-ray scattering.

Protein interactions in undersaturated and supersaturated solutions were investigated using static and dynamic light scattering and small angle x-ray scattering. A morphodrom of lysozyme crystals determined at 35 degrees C and pH = 4.6 was used as a guideline in selecting the protein and precipitant concentrations. The osmotic second virial coefficient, B(22), was determined by static and dynam...

Static Light Scattering Studies on Xanthan in Aqueous Solutions

A commercial sample of xanthan was (i) sonicated and fractionated by GPC on Sepharose Cl-2B with 0.1 M NaCl as eluant/solvent. The angular dependence of scattered light was measured (offline) and interpreted in terms of the wormlike chain model using the “master curve procedure”. The persistence length was estimated as LP≈ 150 nm. The mass per unit lengthML≈ 2000 g/mol·nm indicates double-stran...

Precrystallization structures in supersaturated lysozyme solutions studied by dynamic light scattering and scanning force microscopy

Quasielastic light scattering for protein assembly studies.

Quasielastic light scattering (QLS) spectroscopy is an optical method for the determination of diffusion coefficients of particles in solution. In this chapter, we discuss the principles and practice of QLS with respect to protein assembly reactions. Particles undergoing Brownian motion produce fluctuations in scattered light intensity. We describe how the temporal correlation function of these...